Addicks the latest visitors.U's: Bransgrove, Robinson, Vincent-Young, O'Donoghue, James, Lapslie, Lokko, Harney, Harrison, Szmodics, Curtis, Issa, Lawrence, Bonne, Sembie-Ferris, Smith
The match kicks off at 7pm and is free to Season Ticket holders, including those Six and Twelve Game Pack holders who still have first team games still left on their pack.
There are a number of players in the squad with first team experience this season, and they'll all be looking to impress Tony Humes ahead of the weekend FA Cup fixture.
Fresh from his debut at the weekend at MK Dons, Jamie Harney is included in the squad for the game against Charlton and is likely to line up alongside Kevin Lokko at the heart of the U's defence.
They have drawn their last two games, both away in West London, and return to WHCS looking to make it just one defeat in five matches.
Colchester United's U21s are in action again on Monday night, as they host Charlton Athletic at the Weston Homes Community Stadium.