Conference South trip for Tony Humes' men.
Further details regarding ticket prices will follow in due course, once they have been agreed between the two clubs.
Borough currently sit in 14th place in the table, and have good cup pedigree, having reached the FA Trophy final last season, finishing runners-up.
The U's will take on the Conference South side on the weekend of November 8th and 9th, following this weekend's home match against Port Vale.
Colchester United have been paired with Gosport Borough in the FA Cup after the First Round draw was made on Monday evening.