Birmingham the visitors to Florence Park.
The game kicks off at 1pm and we will have a report on the game later in the day.
It is expected that George Elokobi will play some part in the game as he continues his return to full fitness, whilst Frankie Kent, Drey Wright and Dion Sembie-Ferris all feature.
The U's fell to defeat in their last match against Huddersfield Town, but will get their home fixtures underway at the club's training ground.
Colchester United U21s are back in action on Wednesday afternoon, as they take on Birmingham City at Florence Park.
U's: Triallist, Robinson, Olufemi, Harney, James, Kent, Elokobi, Wilson, Pollard, Lapslie, Sembie-Ferris, Lawrence, Regis, Harrison, Curtis, Dunne, Edge, Wright, Issa
The U's take on Scunthorpe in their next home game. Get your tickets now at the Saver rate - click HERE to buy!