Huddersfield test first up for U's.
The match will also see George Elokobi in competitive action for the first time since his summer switch as he moves closer to full fitness.
Chris Regis and Charley Edge signed for the U's on Friday from Southampton and Everton respectively and will make their U21s league debut on Monday.
The U21s start with a long trip up to Huddersfield, and it will be an early chance for three of our summer additions to make an impression.
The U's first team, U18s and U16s were all in action at the weekend, picking up a win and two draws.
In a busy start to the league season, Colchester United's U21s get their campaign underway on Monday afternoon.
The U's take on Reading in the Capital One Cup and it's not too late to buy your tickets! Book online now at www.colutickets.com!