U's play the first of a home double.
U's: Lewington, Robinson, Olufemi, Burgess, James, Tennent, Brampton, Lawrence, Marlow, Dunne, Harrison, Curtis, Marriott, Bonne, Sheriff
After Tuesday's game, the U21s then host Bristol City at the Weston Homes Community Stadium at 2pm on Thursday.
U21s manager John McGreal will again cast his eye over triallists in the game at Florence Park, with Ben Burgess (Norwich City), Ben Marlow (ex-West Ham) and Decarrey Sheriff (West Ham) aiming to impress the U's management.
The game against the Terriers is the first of two in three days for the U's youngsters as their season enters the final six matches.
Colchester United's latest U21s game sees them face Huddersfield Town at 2pm.