U's face Altrincham on Sunday 6th.
More details on the game, including ticket prices, will follow soon.
The match against the Vanarama National side will kick off at 2pm on the Sunday.
The Second Round game will be one of a number featured on the BBC's Final Score programme on the day, bringing key highlights from each of the matches. Colchester United's Emirates FA Cup game against Altrincham has now been confirmed as taking place on Sunday, December 6th.