U's head to East Thurrock on Wednesday.
The U's squad also includes Femi Akinwande, who is back at the club after a successful loan spell with Bishops Stortford, and recent signing Jermaine Grandison.
The former Fulham and Millwall full back had a hip operation ahead of the start of the new campaign and, after returning to training with the rest of the squad recently, he will play some part in the Quarter Final tie against East Thurrock.
The match sees a return to action for U's defender Matt Briggs, who has been out of action since the final game of last season against Rochdale at the Weston Homes Community Stadium.
Colchester United's young players are back in action on Wednesday evening, as the U's face East Thurrock in the Essex Senior Cup.Williams, Wilson, Briggs, James, Grandison, Peter, Diaz Wright, Regis, Issa, Edge, Brown, Akinwande, Dunne, Cosgrave,
The game kicks off at 7.45pm, and the postcode for East Thurrock's stadium is SS17 9LBMcKeown