The draw for the next round of the Leasing.com Trophy takes place on Saturday
Colchester United are Ball number one in Draw A of the Southern Section, and will play at home.
Home advantage comes the U's way, thanks to topping the table in the group stage.
They beat Gillingham and Ipswich, and drew with Spurs' U21s - securing top spot on seven points.
In Saturday's draw, the U's will be paired with Northampton, Stevenage, Newport or Chelsea U21s, with the tie due to be played the week commencing December 2nd.