Colchester United will play Stevenage in the Second Round of the Leasing.com Trophy
Former U's striker Clinton Morrison and ex-England midfielder Sue Smith made the draw live on Sky Sports Saturday, with thirty two teams left in the competition.
The U's were always guaranteed a home game in the Second Round, having finished top of the group containing League One sides Ipswich Town and GIllingham, along with Spurs U21s.
Second Round games are due to be played in the week commencing December 2nd.
Details for the fixture will be announced in due course.