Colchester United FITC Development Centre player Callum Milton will be representing the U's tonight.
And he will be up against stiff competition with Rotherham United putting forward one of their eSports players to face the U's choice.
The game will kick off at 7pm (or there abouts), and will streamed live on the U's official Twitch page - click HERE to find it.
It is the first round of the tournament organised by fellow League Two side Leyton Orient that has captured the imagination of teams from around the world with clubs from sixteen countries set to take part in the 128 team competition.
Should Callum and the U's progress to the next round, the fixtures will be played from Tuesday evening at 7pm.