After last week's postponement, the young U's side lead by Dave Huzzey and Richard Hall will be on the road this week looking for a win at Cardiff City.
Last week's game against Swansea City was postponed due to the Swans being unable to complete the fixture, meaning the U's have now not had a game for a couple of weeks.
This afternoon though, they will be back in action when they take on Cardiff City, who are the team directly above them in the Professional Development League.
Colchester United currently sit in eighth place, with twelve points, while today's opponents are in seventh with thirteen. It will be seen as an opportunity for the U's who have drawn their last two games.
In their last two, the young U's have played out two 2-2 draws, firstly against Watford at Maldon and Tiptree, then a week later against Ipswich Town at Playford Road.
The hosts for this afternoon's game have won their last two, with a 4-0 win against Ipswich Town and a 2-1 win over Welsh rivals Swansea City.
As always, the U23s games are a valuable source of game time for those struggling to break into the first team, as well as a chance for the youngsters to showcase what they can do.
U's: Johnson, Tchamadeu, Cracknell, Tricker, Akolbire, Marshall Miranda, Cornish, Thomas, Bohui, Lowe, Hutchinson, Stagg, Trialist.