Dave Huzzey's side face the Addicks after victory against Watford in the opening game.
Colchester United's U23 side will take on Charlton Athletic at 7pm this evening at the JobServe Community Stadium in their second match of the Professional Development League season.
Both sides head into the match after taking three points from their first game.
Dave Huzzey's young U's side came out on top againat Watford last weekend with recent first team debutant Sammie McLeod netting the winner in the 1-0 victory away from home.
Tonight's visitors Charlton put three unanswered goals past QPR last weekend with the Addicks sitting just one place above Colchester United in the table.
This evening's match will be played behind closed doors, but will be available to stream at colutickets.com, free for Seasonal Match Permit holders. The stream will start at 6.55pm (5 mins before KO) - click HERE to watch the young U's side in action.
Photo credit: Watford FC