Hayden Mullins' will be searching for their first points of the year on Saturday with Mark Cooper's Barrow making the long journey to North Essex.
Despite the result on Tuesday evening, the U's put in a much-improved performance against Forest Green Rovers and wouldn't have been undeserving of a point at the home of the league leaders.
But games are about goals, and the U's weren't able to find that crucial breakthrough. With a more even opponent on Saturday, as Barrow make the journey to the JobServe Community Stadium.
It was an entertaining game that seemed to include everything earlier in the season, and Colchester United will be hoping that they can do the double over the Bluebirds.
Mark Cooper's side will be going for their first win in five matches, with their last victory against Ipswich Town in the Second Round of the FA Cup in December.
And although last weekend's 5-4 defeat at Championship side Barnsley will give them confidence, Barrow will be hoping they can pick up some points from the U's with both sides sitting just two places apart in the Sky Bet League Two standings.
New signing Jacob Wakeling will be aiming to make an impact after being signed on loan from Premier League Leicester City in the week, after Barrow's third-top scorer, Offrande Zanzala switched to Exeter City on loan.
The Bluebirds' key player, Ollie Banks will be hoping to continue his rich vein of form after netting ten goals in the opening half of the season, including two in his last four games from central midfield.
Cooper has employed a three-man defence for the majority of this season, but has switched his personnel around in the last few games, with different combinations of players used in the last five matches.
One player who has been involved in most of those matches was Matthew Platt, a twenty-four-year-old centre-half who has been with Barrow for the last three seasons.
Last Time We Met
The game at the Dunes Hotel Stadium had it all earlier in the season. The U's went behind, but fought back to take the lead before half time with Alan Judge and Freddie Sears on target.
Luke Chambers was dismissed for a second bookable offence midway through the second half, with Barrow equalising from the penalty spot. But Tom Eastman nodded home the winner ten minutes from time to give Mullins' side all three points.